SILPAKORN UNIVERSITY SCIENCE AND TECHNOLOGY JOURNAL, Vol 9, No 2 (2015)

Somatic Embryogenesis Induction from Protocorm-like Bodies and Leaf Segments of Dendrobium Sonia ‘Earsakul’

Kanjana Juntada, Sudawadee Taboonmee, Pimjai Meetum, Sirinnapa Poomjae, Pantipa Na Chiangmai












Abstract


This study aimed to evaluate effects of types and concentrations of plant growth regulators in a halfstrength Murashige and Skoog medium on somatic embryogenesis from protocorm-like bodies (PLBs) and leaf segments of Dendrobium Sonia ‘Earsakul’ cultured for 8 weeks. It was found that media containing thidiazuron (TDZ) either at 0.1, 0.3 and 1.0 mg/L alone or in combinations with 0.1 mg/L naphthaleneacetic acid (NAA) or 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) provided no significant differences in percentage of somatic embryogenesis (38.18 49%) from PLBs. Half strength MS supplemented with TDZ either at 1 and 3 mg/L alone or combination with 0.1 mg/L NAA provided higher percentage of somatic embryo formation and number of somatic embryo per explant more than supplemented with 0.1 mg/L NAA alone. Low percentage of somatic embryogenesis was found from leaf segments. Leaf explants with the dorsal surface in contact with a medium provided higher somatic embryogenesis (0-18.75%) than those obtained from the explants with the ventral surface contact with culture medium (0.89 8.04%). Furthermore, basal segments of leaves with ventral surface in contact with a medium showed higher somatic embryogenesis and number of embryos (1.79-16.07% and 0.2-0.85, respectively) than those obtained from apical segments (0-1.78% and 0-0.3, respectively). There was a significant difference in somatic embryogenesis (0-18.75%) of a leaf when a dorsal surface was placed on the culture medium.

Key Words: Dendrobium; Plant growth regulators (PGRs); Protocorm-like bodies (PLBs); Somatic embryogenesis

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