SILPAKORN UNIVERSITY SCIENCE AND TECHNOLOGY JOURNAL, Vol 9, No 1 (2015)

Regeneration of Adventitious Shoots from Callus and Leaf Explants in Jatropha curcas L. ‘Phetchaburi’

Pantipa Na Chiangmai, Yupa Pootaeng-on, Pimjai Meetum, Kanokwan Kamkajon, Waraporn Yuiam, Narumon Rungphan, Panwadee Ninkaew
















Abstract


The effect of cytokinins and auxins was evaluated in inducing a physic nut (Jatropha curcas L.) callus while the effect of the combinations of cytokinin and either indole-3-butyric acid (IBA) or some growth additives was determined in regenerating adventitious shoot from a leaf segment. The results showed MS medium containing only 0.1 or 1.0 mg/l thidiazuron (TDZ) was the most effective medium for inducing morphogenic callus. MS medium supplemented with 2.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l indole-3-acetic acid (IAA) gave the highest shoot regeneration percentage and shoot height from the callus. On the other hand, the MS medium supplemented with 0.5 mg/l BA and 1.0 mg/l IBA was the most effective medium for adventitious shoot regeneration and shoot height from the leaf segments.

Key Words: Physic nut; Plant growth regulators; Shoot regeneration; Tissue culture explants

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