SILPAKORN UNIVERSITY SCIENCE AND TECHNOLOGY JOURNAL, Vol 6, No 1 (2012)

Preparation of Pectin from Fruit Peel of Citrus maxima

Uthai Sotanaphun, Amornrut Chaidedgumjorn, Nudchanart Kitcharoen, Malai Satiraphan, Panida Asavapichayont, Pornsak Sriamornsak

Department of Pharmacognosy, Faculty of Pharmacy, Nakhon Pathom

Department of Pharmacetuical Chemistry, Faculty of Pharmacy, Nakhon Pathom

Department of Pharmacognosy, Faculty of Pharmacy, Nakhon Pathom

Department of Pharmacetuical Chemistry, Faculty of Pharmacy, Nakhon Pathom

Department of Pharmaceutical Technology, Faculty of Pharmacy, Nakhon Pathom

Department of Pharmaceutical Technology, Faculty of Pharmacy, Nakhon Pathom


Abstract


The extraction of high-methoxyl pectin from the fruit peel of Citrus maxima was studied. The suitable condition was the extraction at 80 °C without pH adjustment (pH was about 4.5) in 20 times by volume of water. For up-scale procedure, the peel was pre-soaked in water for overnight to wash out small molecular water-soluble substances. Amberlite XAD-16 polystyrene was used to remove phenolic compounds before concentration and precipitation of pectin. This suggested method was simple and inexpensive. The yield of the obtained pectin was 7.23±0.19%. Its galacturonic acid content and degree of esterification were 74.12±2.07% and 76.30 ±3.38%, respectively.

Key Words: Citrus maxima; Galacturonic acid; High-methoxyl pectin; Pomelo; Up-scale production

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