SILPAKORN UNIVERSITY SCIENCE AND TECHNOLOGY JOURNAL, Vol 5 No 1 (January - June) 2011

A Comparison of Image Analysis Software for Quantitative TLC of Ceftriaxone Sodium

Panadda Phattanawasin, Uthai Sotanaphun, Lawan Sriphong, Inthira Kanchanaphibool, Nusara Piyapolrungroj

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom

Department of Pharmacognosy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom

Department of Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom

Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom


Abstract


Three image analysis software, Photoshop, Sorbfil TLC Videodensitometer software and Scion Image, were used for quantitative evaluation of TLC images of ceftriaxone sodium (CFX). Regression plots and detection sensitivity of quantification from each TLC-image analysis were determined and compared to TLC-densitometry. TLC-image analysis method using Scion Image and Sorbfil TLC Videodensitometer software and TLC-densitometry showed the polynomial regression data with good relationship (R2 > 0.99) over the concentration range of 1-8 μg/spot whereas the analysis of TLC images with Photoshop showed good polynomial regression plots (R2 > 0.99) at higher concentration range, 4-10 μg/spot. For detection sensitivity, LOD and LOQ determined from TLC-image analysis using Sorbfil TLC Videodensitometer were comparable to the values from TLC-densitometry. Scion Image and Photoshop was found to be less sensitive. The use of Sorbfil TLC Videodensitometer software for TLC image analysis could be further applied for rapid determination of CFX in bulk drug and dosage forms.

Key Words: TLC; Image analysis software; Ceftriaxone sodium

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