SILPAKORN UNIVERSITY SCIENCE AND TECHNOLOGY JOURNAL, Vol 4, No 1 (2010)

The Effect of Roughage and Urea Solution Infusion Levels on Ruminal NH3-N Concentration and Nutrient Digestibility in Beef Cattle and Swamp Buffaloes

Siwaporn Paengkoum, Metha Wanapat, Chalong Wachirapakorn, Ngarmnit Nontaso

Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi

Tropical Resources Research and Development Center (TROFREC), Faculty of Agriculture, Khon Kaen University, Khon Kaen

Tropical Resources Research and Development Center (TROFREC), Faculty of Agriculture, Khon Kaen University, Khon Kaen

Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen


Abstract


Four, fistulated, male crossbred cattle and swamp buffaloes were randomly assigned in a 2 x 2 factorial arrangement in a 4 x 4 Latin square design. There were four combination dietary treatments; T1) UTS as a roughage and urea infusion at 50 g/h/d (50U), T2) UTS as a roughage and urea infusion at 100 g/h/d (100U), T3) CH as a roughage and urea infusion at 50 g/h/d (50U) and T4) CH as a roughage and urea infusion at 100 g/h/d (100U). Rumen fluid and blood were collected at 0, 0.5, 1, 1.5, 2, 3, 4 and 6 h-post feeding and then analyzed for total bacteria and protozoal, NH3-N, VFA and BUN, respectively. It was found that TDMI in buffoloes fed with UTS as a roughage source with both urea solution infusion were highest (P<0.05). While, digestibility of OM was higher in cattle fed with UTS as a roughage and urea solution infusion in both levels, CP, NDF and ADF digestibility were affected by type of roughage (P<0.05). With regards to digestible nutrient intake, the values of OM, CP, NDF and ADF (kg/d) were significantly different among treatments (P<0.05). Higher levels of ruminal pH were found in buffaloes fed with CH as a roughage and urea infusion in both levels (P<0.05). NH3-N concentrations were higher (P<0.05) in buffaloes fed with CH as a roughage and urea in fusion at 100 g/h/d (34.3 mg%) and lower in cattle fed with both roughage sources and urea infusion at 50 g/h/d (20.0 and 23.5 mg%, respectively). TVFA was affected by type of roughage (P<0.05) and urea infusion at 100 g/h/d. Proportion of C2, C3 and C4 were significantly different (P<0.05) among treatments.

Key Words: Urea infusion; NH3-N, Rumen Microbes; Beef Cattle; Swamp Buffaloes; Digestibility

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