SILPAKORN UNIVERSITY JOURNAL OF SOCIAL SCIENCES, HUMANITIES, AND ARTS, Vol. 3 (Number 1-2) 2003

Multiple Shoot Induction and Plant Regeneration of Rosa damascena Mill

Chitribul Bhoomsiri, Nalinrut Masombo






Abstract


An in vitro regeneration system was developed for
propagation of Rosa damascena Mill. Axillary shoot proliferation
and root induction were induced in the nodal segments on Murashige
and Skoog (MS) medium and Quoirin and Lepoivre (QL) medium
supplemented with benzyladenine (BA) or รก-napthalene acetic acid
(NAA) or in combination within 4 weeks. The highest number of
root per explant was obtained from MS medium supplemented
with 0.5 mg/l NAA and 87.5 % of the explants exhibited root
development, 2.71 roots per explant. The best response in terms of
frequency of shoot regeneration (100%) and maximum number of
shoots (8.27 shoots per explant) were developed on QL medium
containing a combination of 0.1 mg/l NAA and 4.0 mg/l BA. A
proliferating shoot culture was established by repeatedly
subculturing newly formed shoots at 4 week intervals. Thus, 59.8
shoots were obtained in 8 weeks from a single nodal explant.
Rooting of the regenerated shoots was induced on MS medium
supplemented with 0.5 mg/l NAA and 85% of the shoots
developed roots at an average of 2.3 per shoot within 4 weeks.
Ninty percent of plantlets were acclimatized and established in soil.

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