Preliminary Study on the Effect of Culture Medium on the Number and Size of Endospores of Bacillus megaterium
Ashara Pengnoo, Ruedeekorn Wiwattanapattapee, Amornrat Chumthong, Wanit Rotniam, Mana Kanjanamaneesathian
Bacillus megaterium, an effective bacterial antagonist against Rhizoctonia solani, was cultured in the media prepared from cheap agricultural substrates. Number of bacterium and size of endospores of B. megaterium were assessed after culturing the bacterium in the broth extracted from potato (Solanum tabersosum), cassava root (Manihot esculenta Crantz), sweet potato (Impomoea batatasil (L.) Poir), rice (Oryza sativa), brown rice (O. sativa), sticky rice (O. sativa), and Job’s tears (Coix lachryma jobi L.). The highest number of endospores of B. megaterium was obtained when the bacterium was cultured in broth medium prepared from brown rice. However, after 3 months storage, number of the bacterium cultured in cassava root remained highest. Bacterium cultured in broth medium prepared from sticky rice provided the highest width of endospores. Nevertheless, types of substrates used for broth medium preparation had no effect on length of the endospores.
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